
Nathan Shaner / MBARI
This tropical postcard consists of a petri dish containing an artistic arrangement of bacteria that have been genetically engineered to incorporate fluorescent proteins.
Microbiologists are starting to make sense of tens of thousands of samples they've collected from around the world, undoubtedly containing legions upon legions of different kinds of microorganisms. How many kinds? That's just the point: Nobody knows.
The microbial world is "Earth's dark matter," says Janet Jansson, a senior staff scientist at Lawrence Berkeley National Laboratory in California. By that, Jansson means that the varieties of bacteria and other microorganisms are as mysterious as the unseen stuff that makes up 85 percent of the matter in the universe.
Jansson held up a spoon of soil during a news conference Friday at annual meeting of the American Association for the Advancement of Science, held in Vancouver, Canada — and noted that there were more organisms in that spoonful than there were stars in the Milky Way galaxy (100 billion).
Talk about big numbers: Scientists estimate that there are 10 trillion microbes in every kilogram (2.2 pounds) of soil on Earth. Our planet is home about a nonillion cells (that's a 1 with 30 zeroes after it). Most of those are microbes. Each human body is thought to consist of 10 trillion cells, harboring microbial communities that amount to 100 trillion cells. From a microbe's point of view, we're all just lumps of flesh that are convenient places to hang out, said Jack Gilbert, a microbiologist at Argonne National Laboratory and the University of Chicago.
"Without them, you'd be dead," he told reporters at an AAAS meeting. "Without us, they'd just move onto something else."
Earth Microbiome Project
The problem is that far less than 10 percent of the world's varieties of microbes have ever been cultivated in the lab. The rest are out there in the world, beyond the reach of the traditional methods for categorizing and analyzing life forms. That's where the Earth Microbiome Project is aiming to make a big difference.
Over the past year, more than 100 researchers have been collecting samples from locales as far-flung as the Antarctic Dry Valleys, the Great Indian Desert, Yellowstone's hot springs and a Merlot grape vineyard on Long Island. Swabs have been sent in to document the microbial communities living within ants, iguanas and other animals. The effort meshes with the Human Microbiome Project, a longer-running, federally financed campaign to study the microbes living in us and on us.
Jansson said about 60,000 samples have been collected during the first year of the Earth Microbiome Project, and about 10,000 of those samples have been processed. Rather than trying to culture individual bacteria, the microbe-hunters are doing wholesale DNA sequencing to piece together as many genomes as they can. Eventually, the project's organizers hope to analyze hundreds of thousands of samples.
A few more samples are being sent in this week, courtesy of the journalists attending Friday's news briefing. Following the researchers' instructions, we swabbed our smartphones as well as the soles of our shoes, popped the swabs in collection vials, and handed them over to students for analysis over the next few weeks. I'll let you know if I find out anything interesting about the microbial communities living in my pants pocket or on my slip-ons.
Eventually, the project plans to produce a microbial gene atlas as well as a "field guide" to microbes from regions around the world, said Jonathan Eisen, a microbiologist at the University of California at Davis.
What's this have to do with us?
Insights into Earth's microbial dark matter could yield all sorts of benefits for science and our well-being. First of all, shedding light on the planet's microbial dark matter will give scientists a better sense of how Earth's "tree of life" is laid out. Just in the first year, the project has covered 82 percent of the currently known global diversity of microbes, Gilbert reported.
Charting the human microbiome should be a particularly fruitful exercise. Rob Knight, a researcher at the University of Colorado at Boulder, showed off some visualizations illustrating how the microbial communities on our fingertips, our face and in our mouths have distinct characteristics that can be charted over time — theoretically revealing where our fingers have been. "This raises all kinds of ethical concerns," he said, only half-jokingly.
Studies have shown that babies delivered vaginally and through Caesarian section have significantly different microbiomes 20 minutes after birth, Knight said. That could mean that those babies face different prospects for immune responses and allergies later in life — prospects that could be changed by postnatal "inoculations" with the right kind of bacteria.
Gilbert said fecal samples could reveal how our gut bacteria are doing, and whether we need to have our microbiomes adjusted for better health. As icky as it might sound, fecal transplants have already become an accepted therapy for some types of intestinal infections.
Even the microbiomes that have nothing directly to do with humans could be important. Take that Merlot vineyard, for example. "We're doing the microbiome of a 'good year,'" Gilbert said. If for some reason the wine made from the vineyard's grapes becomes less tasty, it might be possible to load up the soil with the good-year bacteria and restore the vintage to its glory days.
Gilbert would like to see the Earth Microbiome Project get to a point where it's possible to predict future changes in the ecosystem — including climate change impacts — by checking something like a microbial "weather report." But to do that, researchers will have to manage massive amounts of genomic and environmental data. That's a challenge that Rick Stevens, the Argonne Lab's associate director, compared to unraveling the secrets of subatomic particles with the Large Hadron Collider.
To study the smallest life forms on the planet, "we need bigger, better computers," he said.
Is this a job worth doing? The scientists leading the Earth Microbiome Project definitely think so. "I think people should be excited about this," Gilbert said. Are you excited? Feel free to weigh in with your comments below.
More from the AAAS meeting in Vancouver:
- Device turns gestures into song
- Researchers working to build a better leaf
- Answers ahead for physics' deepest mysteries
- Scientists revive sounds of Stonehenge and other sacred spaces
- Gas-drilling gaffes aren't unique to fracking, study says
Alan Boyle is science editor at msnbc.com. Connect with the Cosmic Log community by "liking" the log's Facebook page, following @b0yle on Twitter or adding the Cosmic Log Google+ page to your circles. You can also check out "The Case for Pluto," my book about the controversial dwarf planet and the search for new worlds.


While it seems a worthwhile endeavor in its own right, some of the microbes should be cultured and observed. Especially any ones related to useful organisms or any that just seem neat. You never know what those neat microbial enzymes might be capable of.
Happy hunting microbiologists! Just beware. After all, you are outnumbered by your prey. Really, really outnumbered!
Absolutely. Their genetic codes and those of other multicellular life on the planet can be invaluable for us humans once we actually bother to grasp their uniqueness.
As one of the people quoted in the article I appreciate your comment Terror Bird and I note - culturing and characterizing as many organisms as possible is part of what I discussed in my talk on "A field guide to the microbes". The only issue with this is that many of the microbes on the planet cannot be grown in the lab right now. An alternative to getting enzymes from them is to isolate DNA directly from the environment and to characterize the genomes of these organisms.
can we deduct all those critters as dependants to the irs?
Heh-heh!
Yes I'm excited as hell as long as we aren't paying for it. Sending people globe trotting to stick shovels full of dirt in bags and bring them back is probably important but, and please don't let this get out, WE'RE FRIGGING BROKE. It's very likely that they would cut social security and let our seniors starve before they would cut funding for something like this. Anyway I'm sure we're not paying for it because it wasn't mentioned in the story. Oh wait ..
I am very glad that our nation and its taxpayers are sponsoring this vital research. It is unfortunate that many common people do not understand the gravity of this microbial exploration, but believe me, when you're dying from a disease and drugs made by these critters are your only lifeline, or when these critters are making the next gen biofuels, perhaps you will then know, or perhaps you still won't.
Let me just put it this way - it's best to not talk about things you're wholly ignorant about, lest you make a complete fool out of yourself. Read the comment below by Keira Desbiens. You showed just how low you could get by not wanting to understand 91% of the cells in your body.
Don't want them germs jetting around the world and living it up in fancy petri dishes on MY dime! They can get a job!
You can request a DIME refund from the IRS. Just don't expect any help if they discover some cure for a fatal disease that you might contract.
Life begins where the pavement ends.
The numbers mentioned in the article are staggering. If microbial life has, or still does, exist on Mars as prolifically as here on earth we should be able to find it. The data from the upcoming Mars Curiosity mission will be fascinating.
Doug - Their job is exactly what we need to figure out. We need to sort the good from the bad, carefully, and try to surround ourselves with the good and stave off the bad. These critters may have more to do with how long you live than how many pounds of bacon you eat.
The reason you go broke is that you don't invest in these things. A great part of the current US technology base is based on basic research of various kinds from the 40s to the 90s. This one can be especially fruitfull since it potentially impacts a great number of fields. Considering that one day of spending in Afganistan probably covers the cost of this project a hundred times, I find the freaks against this kind of spending particularly specious.
Keira @8, I agree. Is it a job worth doing and exciting? Yes and yes! Please, more research and knowledge! The possibilities for medicine and useful products is amazing.
"Alan Boyle" .... This topic is under rated ....
Single celled organisms should be our biggest fear , due to their ability to mutate quicker than anything else , from their ability to reproduce at such a rapid rate ....
I could go on and on about their potential danger ....
Another great topic .... Thanks ....
Considering 10 TRILLION live on just your body alone (Sorry for grossing out all of you with that true statement) ......thats trillions upon trillions upon trillions upon trillions upon trillions on the planet earth.
Who has really conquered the planet here? Its not us thats for sure.
They are the 99.99999999999999999999999 %
It appears we are all tied together in a subliminal way... :-)
Midi-chlorians are a microorganism first mentioned in Star Wars Episode I: The Phantom Menace. They are microscopic life-forms that reside within the cells of all living things and communicate with the Force.[6] They are symbionts with all other living things and without them life could not exist. The Jedi have learned how to listen to and coordinate the midi-chlorians. While every living being thus has a connection to the Force, one must have a high enough concentration of midi-chlorians in one's cells in order to be a Jedi or a Sith.[7][8]
Creator George Lucas says that the midi-chlorians are based on the endosymbiotic theory
Earth's "tree of life" ?
Life: I wonder where that came from??
'Life' is just a word used to denote self-replicating units of carbon-based chemistry. It's kind of a figurative term, like 'God.' You don't want to take them literally....
I don't think it is always carbon-based, but that makes it even more interesting.
The idea that humans are, in reality, little more than walking petri dishes is humbling; it takes us down the food chain from apex predator to the same level as cattle and chickens.
Sol 1's comment about midi-chlorians raises a valid point- how much human behavior might be microbial behavior...perhaps humans unwittingly congregate in towns and citiies to exchange microbes, and label that urge as social contact.